Coding

Part:BBa_K1104200:Experience

Designed by: Ting-Yun Chiang   Group: iGEM13_NYMU-Taipei   (2013-09-11)
Revision as of 13:39, 27 October 2013 by Windy6632 (Talk | contribs) (→‎Applications of BBa_K1104200)

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SDS-PAGE

We conduct an SDS Polyacrylamide gel electrophoresis for OxyR expression.

NYMU SDS.png

Setting control group contains only E.coli MG1655 and experimental group with K1104250 added to AhpCp2D1(K1104204), the electrophoresis result shows OxyR (Molecular Weight of Polypeptide: 34.276 kD) can really be expressed.

Applications of BBa_K1104200

OxyR-included circuit(Device1+Device2)

In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1(Part:BBa_K1104250).

Device1 in order to enhance the effect of ROS on E. coli is composed of :

  1. a constitutive promoter(Part:BBa_J23102)
  2. RBS(Part:BBa_B0034)
  3. activator OxyR(Part:BBa_K1104200)
  4. double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
Device1
a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015
Device2
a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840)

Putting Device1 BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.

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