1 Registry Star
Generator
BSMT1
Part:BBa_J45119:Experience
Designed by: Boyuan Zhu Group: iGEM06_MIT (2006-08-10)
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Applications of BBa_J45119
- Wintergreen odor based reporter of transcriptional signal.
User Reviews
UNIQa237a32f6985bfc8-partinfo-00000000-QINU
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BBa_J45119 was successfully used to produce wintergreen odor under the control of BBa_R0040 in the wintergreen odor generator BBa_J45120. |
UNIQa237a32f6985bfc8-partinfo-00000005-QINU
Transcriptional control of wintergreen odor generator
Growth phase dependent wintergreen odor production
To demonstrate growth phase dependent wintergreen odor production, we compared the behavior of constitutive and exponential phase dependent wintergreen odor generators (A and B, respectively). We measured methyl salicylate concentration (relative to the pentachloronitrobenzene internal standard) of cultures of the constitutive and exponential phase wintergreen odor generators at different culture cell densities (OD600nm) (C). The constitutive and exponential phase wintergreen odor generators produced similar levels of methyl salicylate at all cell densities examined. Thus, the exponential phase wintergreen odor generator does not work as intended.
To demonstrate growth phase dependent wintergreen odor production, we compared the behavior of constitutive and exponential phase dependent wintergreen odor generators (A and B, respectively). We measured methyl salicylate concentration (relative to the pentachloronitrobenzene internal standard) of cultures of the constitutive and exponential phase wintergreen odor generators at different culture cell densities (OD600nm) (C). The constitutive and exponential phase wintergreen odor generators produced similar levels of methyl salicylate at all cell densities examined. Thus, the exponential phase wintergreen odor generator does not work as intended.