Device

Part:BBa_K1065102:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-08-28)
Revision as of 11:21, 3 October 2013 by Cate (Talk | contribs) (→‎References)


Wintergreen device: Plac + PchBA + AraCpBAD + BSMT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
    Illegal AgeI site found at 2942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924


Design Notes

The part was created subcloning BBa_J45320 inside BBa_K1065101 following the standard assembly protocol.

Source

PchBA were extracted from Pseudomonas aeruginosa. AraC-pBAD was extracted from E. coli. BSMT1 was extracted from Petunia x hybrida.

References

1. Marianne C. Verberne at al., (1999). Salicylic acid biosynthesis, Biochemistry and Molecular Biology of Plant Hormones, 295–312.
2. Florence Negre at al., Novel S-adenosyl-l-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers, Archives of Biochemistry and Biophysics, Volume 406, Issue 2, 15 October 2002, Pages 261–270.