Composite

Part:BBa_K1065306:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)
Revision as of 20:20, 28 September 2013 by Fabius924 (Talk | contribs) (→‎Design Notes)

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J23100+YF1+FixJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

This part was built starting from the extraction of BBa_K592016 from the iGEM Registry 2013 Distribution Kit. The part then was subcloned in BBa_J23100 following standard assembly.

Source

This part has been built using:
BBa_J23100 a constitutive promoter from the Anderson family.
BBa_K592016 the part with RBS-coupled YF1 and FixJ.

References

1) Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.
2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542