Composite

Part:BBa_K1065306:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)
Revision as of 09:37, 28 September 2013 by Cate (Talk | contribs) (→‎References)


J23100+YF1+FixJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

This part was built by starting from the extraction of BBa_K592016 from the iGEM Registry 2013 Kit Distribution, then it was subcloned in BBa_J61002 by standard assembly.

Source

This part has been built using tow:
BBa_J23100 a constitutive promoter from the Anderson family.
BBa_K592016 the part with RBS-coupled YF1 and FixJ.

References

1Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology,Pages 1433–1444.