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Coding
TALEA-dife
Part:BBa_K1189021
Designed by: Chris Wintersinger, Denny Hoang, Taylor Remondini Group: iGEM13_Calgary (2013-09-17)
TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker
This part was created by the fusion of the heavy and light ferritin subunits (BBa_K1189025, BBa_K1189024) to form the fusion ferritin subunit. The lacI promoter (BBa_J04500), double terminator (BBa_B0015) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to BBa_K1189018, but instead of the E-coil, it is replaced by TALA(BBa_K1189004). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3656
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Categories
Parameters
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