Composite
Part:BBa_K1036005:Design
Designed by: Zhaopeng Cheng, Xiyu Wu Group: iGEM13_XMU-China (2013-09-09)
sfgfp (with LVA-tag) under plux R control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2737
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
Illegal BsaI.rc site found at 2017
Illegal SapI.rc site found at 31
Design Notes
it is used to construct sfGFP
Source
The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.
References
1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)