Part:BBa_K1073020:Experience

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Applications of BBa_K1073020

When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

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••••• iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined according to these spectra. iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. Absorption maximum for aeBlue: 595 nm iGEM Team Braunschweig 2013:Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. Emission maximum for aeBlue: none (dark quencher) AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012]. According to these results the optical density for growth curves of cells expressing this protein was measured at 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.

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