Device
Part:BBa_K1150024:Design
Designed by: L. Schmunk, P. Schwenk Group: iGEM13_Freiburg (2013-09-17)
uniCAS Histone Modifier (CMV promoter)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 900
Illegal BglII site found at 5375 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker seperates the dCAS9 and the G9a-SD to minimize the possibility of interference in between the domains.\\
Source
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