Device

Part:BBa_K1150024:Design

Designed by: L. Schmunk, P. Schwenk   Group: iGEM13_Freiburg   (2013-09-17)

uniCAS Histone Modifier (CMV promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 900
    Illegal BglII site found at 5375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker separates the dCas9 and the G9a set-domain to minimize the possibility of interference between the domains. Freiburg2013 Plasmid Cas9-G9a.png
Figure 1.: Complete overview on CMV:dCas9-G9a with all features.

Source

..

References