Coding
Part:BBa_K1189031:Design
Designed by: Ali Honarmand, Somshukla Chaudhuri Group: iGEM13_Calgary (2013-09-17)
TALE-A linked to beta-lactamase with a his tag under a lacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2651
Illegal AgeI site found at 677
Illegal AgeI site found at 1258 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was generated synthetically by Genscript and optimized for E. coli K12. As opposed to the eukaryotic Kozak seuquence on BBa_782004 TALE A from which the TALE in this sequence was inspired, the iGEM Calgary 2013 team placed a strong prokaryotic (B0034) RBS to express it.
Source
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