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Part:BBa_K1184000:Experience
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Applications of BBa_K1184000
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Carnegie_Mellon 2013 |
![]() Figure 1: Phototoxicity of KillerRed and mRFP1 (BBa_E1010) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours. KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1. XL10 Ultracompetent cells were transformed with BBa_K1184000 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.
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