Coding
SAMT

Part:BBa_J45001:Design

Designed by: Kate Broadbent   Group: iGEM06_MIT   (2006-06-07)
Revision as of 15:51, 26 June 2006 by Drephd (Talk | contribs) (→‎Design Notes)


SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 471
    Illegal SapI.rc site found at 75


Design Notes

WE WANT TO REORDER: the reverse and the internal sequencing primers (6/26)

  1. no restriction enzyme cut sites in coding region
  2. changed natural stop codon to TAA TAA
  3. primer design
    • forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'
    • reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'
    • internal sequencing primer = 5' - gga atg ctg cta tat ttg gg

Source

  1. GenBank Accession No. AF515284
  2. Species = Antirrhinum Majus, Snapdragon

References

  1. corresponding author: Natalia Dudareva
  2. Pubmed: PMID: 12361714
  3. Abstract for SAMT enzyme:
    1. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12361714&dopt=Abstract
  4. Wiki Link:
    1. http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6