Part:BBa_J45001:Design

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 577
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 901
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 425
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 471
  Illegal SapI.rc site found at 75
  

Design Notes

• No BioBrick restriction enzyme cut sites in coding region
• Changed natural stop codon to TAA TAA
• Constructed by PCR

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'

Internal sequencing primer

5'- gga atg ctg cta tat ttg gg -3'

Source

SAMT gene from Antirrhinum majus (snapdragon flower) ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 GenBank AF515284])

References

<biblio>

1. Negre-ArchBiochemBiophys-2002 pmid=12361714

</biblio>

• [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]