Composite

Part:BBa_K1149020:Design

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-08)
Revision as of 23:37, 8 September 2013 by Margarita K (Talk | contribs) (→‎Design Notes)


Promoter J23104 - RBS B0034 - amilCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a composite part from BBa_K608002 (https://parts.igem.org/Part:BBa_K592009) and BBa_K592009 (https://parts.igem.org/Part:BBa_K608002). It is functionally similar to BBa_K592031 (https://parts.igem.org/Part:BBa_K592031) which has a J23101 promoter instead of the J23104 but contains the same RBS and amilCP.

There is an extra G after the prefix and there are 2NCs missing in the scar site.

Source

References

Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.