Translational_Unit
AtIPT7

Part:BBa_K1139101:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-08-29)
Revision as of 11:49, 26 September 2013 by Keso57 (Talk | contribs) (→‎References)


Adenylate isopentenyltransferase 7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882


Design Notes

The cds is exactly the same as IPT7 gene sequence of Arabidopsis thaliana. The RBS is [1] (efficiency 0.6).

Source

http://www.ncbi.nlm.nih.gov/gene/821943

References

1. Kentaro Takei, Hitoshi Sakakibara1, Mitsutaka Taniguchi and Tatsuo Sugiyama. (2001) Nitrogen-Dependent Accumulation of Cytokinins in Root and the Translocation to Leaf: Implication of Cytokinin Species that Induces Gene Expression of Maize Response Regulator. Plant Cell Physiol, 42, 85–93
2. Kentaro Takei, Hitoshi Sakakibara, and Tatsuo Sugiyama. (2001) Identification of Genes Encoding Adenylate Isopentenyltransferase, a Cytokinin Biosynthesis Enzyme, in Arabidopsis thaliana. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 276, 26405-26410