Generator
ccdB

Part:BBa_P1011:Design

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-03-13)
Revision as of 23:09, 17 August 2006 by Rshetty (Talk | contribs)

ccdB cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is derived from BBa_P1010.

The following changes have been made.

  1. ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.
  2. Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
  3. Silent mutations have been introduced in order to eliminate useful restriction sites and rare codons.

Source

BBa_P1010

References