Part:BBa_K734000

Caffeine demethylation pathway

Synthetic operon consisting of methylxanthine N-demethylases: NdmA,NdmB, NdmC, and associated reductase NdmD from Psuedomonas putida CBB5. Also includes gst9 from Janthinobacterium sp. Marseille which is required for NdmC functionality. Constitutive expression is driven by promoter BBa_J23100.

Enables Escherichia coli cells to degrade caffeine (1,3,7-Methylxanthine) and related methylxanthines to xanthine.

See 2012 UT Austin wiki: [http://2012.igem.org/Team:Austin_Texas/Caffeinated_coli Caffeinated coli]

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1229
  Illegal XbaI site found at 1126
  Illegal XbaI site found at 2229
  Illegal XbaI site found at 3109
  Illegal PstI site found at 548
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1229
  Illegal NheI site found at 8
  Illegal NheI site found at 31
  Illegal NheI site found at 1730
  Illegal NheI site found at 1925
  Illegal PstI site found at 548
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1229
  Illegal BglII site found at 2687
  Illegal BglII site found at 3181
  Illegal BglII site found at 3652
  Illegal XhoI site found at 4793
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1229
  Illegal XbaI site found at 1126
  Illegal XbaI site found at 2229
  Illegal XbaI site found at 3109
  Illegal PstI site found at 548
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1229
  Illegal XbaI site found at 1126
  Illegal XbaI site found at 2229
  Illegal XbaI site found at 3109
  Illegal PstI site found at 548
  Illegal NgoMIV site found at 5491
  Illegal NgoMIV site found at 5604
  Illegal AgeI site found at 2224
  Illegal AgeI site found at 3320
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 984
  Illegal BsaI.rc site found at 2221