Coding
Part:BBa_K902032:Design
Designed by: Jeff Addison Group: iGEM12_Calgary (2012-10-02)
Revision as of 04:23, 3 October 2012 by Jeffaddison89 (Talk | contribs)
CarAa Coding Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct.
Source
Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template