Coding

Part:BBa_K902032:Design

Designed by: Jeff Addison   Group: iGEM12_Calgary   (2012-10-02)

CarAa Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct.


Source

Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template

References

Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).