Part:BBa_K823030

P_cotYZ: B. subtilis promoter regulating expression of spore crust proteins

P_cotYZ: Promoter of B. subtilis that is involved in spore crust formation. Hence it is activated in late sporulation. P_cotYZ regulates the crust protein cotZ and cotY.

CotVWXYZ cluster. (modified after [http://www.ncbi.nlm.nih.gov/pubmed/7519271 Zhang et al., 1994)]

We used the promtoer P_cotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

These are results of a plate reader assay to evaluate promoter activity. P_cotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter P_cotV BBa_K823033 with each two clones.

These are results of a plate reader assay to evaluate promoter activity. P_cotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV (BBa_K823033) with each two clones.

This part was used to display fusion proteins on B. subtilis spores with [[1]]. 100px|link=https://parts.igem.org/Part:BBa_K823049

See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]