Plasmid_Backbone

Part:pSB1AC3

Designed by: Tom Knight   Group: iGEM05_Registry_Example   (2005-06-07)
Revision as of 02:48, 9 June 2007 by Smelissali (Talk | contribs)

High copy BioBrick assembly plasmid

pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

Annotated APE file for pSB1AC3
Plasmid map

Usage and Biology

Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3040
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3034
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3034
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2073


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
originpMB1
resistanceAC