Composite

Part:BBa_K823052

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-09-10)
Revision as of 09:13, 26 September 2012 by Jara (Talk | contribs)

lacZ, B. subtilis optimized + RBS + Term

lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site and terminator (B0014).

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.

This BioBrick was used for promoter evaluation in B. subtilis.

Find out more about the design of our prefix with ribosome binding site.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT


We used this lacZ gene to evaluate our promoters PliaG, PliaI, Pveg with β-galactosidase-assays for B. subtilis.

Find out more about our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks Bacillus BioBrick Box]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None