Part:BBa_K934012:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K934012
To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa_S03119) and Plux-GFP (BBa_K395100) to E.coli as a “Lux reporter cell”.
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI (BBa-K934012) synthesized 3OC6HSL.
We accomplished a positive feedback system with our new Plux-LasI (BBa_K934022).
By co-culturing Plux-LasI cell and Plas-LuxI cell, we confirmed higher concentration of a signal than initial conditions was detected through production of the other signal (red arrow & blue arrow). This result strongly suggests that our positive feedback system worked accurately.
User Reviews
UNIQ5d613fcab66f2ee2-partinfo-00000000-QINU UNIQ5d613fcab66f2ee2-partinfo-00000001-QINU