Composite

Part:BBa_K934012:Experience

Designed by: Toshiki Hashimoto   Group: iGEM12_Tokyo_Tech   (2012-09-17)
Revision as of 19:13, 25 September 2012 by Toshiki (Talk | contribs) (→‎Applications of BBa_K934012)

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Applications of BBa_K934012

Plas-LuxI result.png


To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa_S03119) and Plux-GFP (BBa_K395100) to E.coli as a “Lux reporter cell”.

In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI (BBa-K934012) synthesized 3OC6HSL.

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