Composite

Part:BBa_K929003:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-18)
Revision as of 19:07, 25 September 2012 by ChristopherKressler (Talk | contribs)

modified AID with CMV, hGH-polyA and eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2360
    Illegal BsaI.rc site found at 770
    Illegal BsaI.rc site found at 1887
    Illegal SapI site found at 871


Design Notes

UP12 Designinfo BBa K929003.png

This part was made by cloning via RFC 10 cloning standard. The parts "modified AID+eGFP" (BBa_K929004);"CMV" (BBa_I712004)and hGH polyadnylation sequence (BBa_K404108) were serial cloned together. For information how "modified AID+eGFP" (BBa_K929004) was designed see its DesignPage.

Source

4 parts: CMV promoter (BBa_I712004), superAID(BBa_K929001), eGFP (BBa_K404316) and hGH polyadenylation signal sequence (BBa_K404108)

References