Part:BBa_K929003:Design

modified AID with CMV, hGH-polyA and eGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1958
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2360
  Illegal BsaI.rc site found at 770
  Illegal BsaI.rc site found at 1887
  Illegal SapI site found at 871
  

Design Notes

This part was made by cloning via RFC 10 cloning standard. The parts "modified AID+eGFP" (BBa_K929004);"CMV" (BBa_I712004)and hGH polyadnylation sequence (BBa_K404108) were serial cloned together. For information how "modified AID+eGFP" (BBa_K929004) was designed see its DesignPage.

Source

3 parts: "modified AID+eGFP" (BBa_K929004);"CMV" (BBa_I712004)and "hGH polyadnylation sequence" (BBa_K404108)