Coding
Part:BBa_K864200:Design
Designed by: Erik Lundin Group: iGEM12_Uppsala_University (2012-09-25)
MarR native E. coli repressor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below (Annealing sequences are underlined)
marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT
marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT