Coding

Part:BBa_K864200:Design

Designed by: Erik Lundin   Group: iGEM12_Uppsala_University   (2012-09-25)

MarR native E. coli repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined)

marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT

marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT

References