Composite

Part:BBa_K733012:Design

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 17:58, 22 September 2012 by Wyq.hkust (Talk | contribs) (→‎Source)

xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.

Source

We digest and ligate our xylR+PxylA+RBS+ydcE and pTms+RBS+ydcD.

Backbone: pTms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI.

Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification.

References