Composite

Part:BBa_K733011

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)

xylR+PxylA+RBS+ydcE.

This constructs contains a xylose inducible promoter with a toxin gene – ydcE (ndoA). Upon the induction of xylose, the cell will express EndoA from ydcE, which will be harmful to the bacteria and inhibit cell growth. For the explicit information of the xylose promoter and ydcE, please click:

xylR+PxylA: BBa_K733002

ydcE: BBa_K733004

Cell Growth Inhibition Device: BBa_K733012


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None