Part:BBa_K808000

araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter.

Usage and Biology

L(+) - Arabinose is a sugar and ist harmless. For overexpression of proteins the concentration of 0,001-0,02% Arabinose can be used.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

This sequenced biobrick was alingned by using nucleotide NCBI BLAST with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.

Reference

Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565.