Regulatory
Part:BBa_K861174
Designed by: Xian Xia Group: iGEM12_WHU-China (2012-09-08)
A control device for BBa_K861169
This is an intermediate device for function detection of the indirect regulatory device K861169. In the device, a constitutively expressed cI is assembled with promoter repressed by cI, resulting in repression of the gene downstream. In comparison with the device K861169 which is activated by glucose, RFP in this device will not be expressed at any glucose concentration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1611
Illegal AgeI site found at 1723 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | A control device for BBa_K861169 |