Regulatory

Part:BBa_K861174

Designed by: Xian Xia   Group: iGEM12_WHU-China   (2012-09-08)

A control device for BBa_K861169

This is a control device for function detection of the indirect regulatory device K861169. In the device, a constitutively expressed cI is assembled with promoter repressed by cI, resulting in repression of the gene downstream. In comparison with the device K861169 which is activated by glucose, RFP in this device will not be expressed at any glucose concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1611
    Illegal AgeI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aA control device for BBa_K861169