Composite
Part:BBa_K784009
Designed by: Ilya Vainberg Slutskin Group: iGEM12_Technion (2012-09-15)
Spacer2+RBS+mCherry
This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.
Due to a mutation, this part differs from BBa_K784007.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |