Part:BBa_K784006

Theophylline riboswitch 12.1 + mCherry

This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 15
  
• 1000
  COMPATIBLE WITH RFC[1000]