RNA

Part:BBa_K784005

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-13)
Revision as of 18:15, 13 September 2012 by Sl.ilya (Talk | contribs)

Theophylline riboswitch 12.1

A riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur. This part is a duplicate of BBa_J89000 but with additional sequence notations and additional sequence upstream to the theophylline aptamer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None