Part:BBa_K823032
cotZ: B. subtilis spore crust protein
This part codes for one of the outer most spore crust proteins in B. subtilis. We designed this part in the Freiburg standard (Assembly 25) to use it as a platform for protein display on B. subtilis spores.
The aim of the LMU-Munich 2012 project is to create spores that display fusion proteins on their crust. There are several different proteins forming the spore coat layers of Bacillus subtilis spores. On the outermost layer, the so called spore crust, the CotZ and CgeA proteins are located ([http://www.ncbi.nlm.nih.gov/pubmed?term=imamura%20et%20al.%202011%20spore%20crust Imamura et al., 2011]). This is why we used them to create functional fusion proteins to be expressed on our Sporobeads.
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The cluster cotVWXYZ contains the gene cotZ which is cotranscribed with cotY regulated by the promoter PcotYZ.
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We were finally able to display GFP on B. subtilis spores by using cotZ (see device BBa_K823049).
NOTE: There are two versions of the cotZ gene in the registry, BBa_K823031 (cotZ -2aa, this part) and BBa_K823032 (cotZ), for our purpose cotZ -2aa, worked better.
See all the details about the LMU-Munich 2012 project [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins here].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 48
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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