Plasmid_Backbone
pSBBs3C
Part:BBa_K823025
Designed by: Jara Radeck Group: iGEM12_LMU-Munich (2012-08-21)
pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays.
This backbone is a BioBricked version of the B. subtilis vector pAH32.
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]
Reference: [http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch et al.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 10619
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 10625 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 10619
Illegal BglII site found at 5170
Illegal BamHI site found at 5705 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 10619
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 10619
Plasmid lacks a suffix.
Illegal XbaI site found at 10634
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 9536
Illegal AgeI site found at 576 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 7576
Illegal BsaI.rc site found at 3489
Illegal SapI site found at 1095
Illegal SapI.rc site found at 8658
[edit]
Categories
Parameters
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/libraryscreening/promoter
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/libraryscreening/promoter
chassis | B. subtilis + E. coli |
copies | integrative in B. subtilis |
origin | pAH328 |
resistance | A(E. coli) + C(B. subtilis) |