Regulatory
Ara

Part:BBa_K808000

Designed by: Valentina Herbring, Sebastian Palluk, Andreas Schmidt   Group: iGEM12_TU_Darmstadt   (2012-08-11)
Revision as of 16:55, 22 September 2012 by A.Schmidt (Talk | contribs)

araC-Pbad - Arabinose inducible regulatory promoter/repressor unit


This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter. L(+) - Arabinose is a sugar and is harmless. For overexpression of proteins the concentration in range of 0,001-0,02% Arabinose can be used.

This sequenced biobrick was alingned by using nucleotide NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_SPEC=WGS&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch) with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/other
Parameters
biology
controlaraC, arabinose
directionForward
n/aInducible pBad/araC promoter
negative_regulators
o_h
o_l
positive_regulators1