Regulatory
Ara
Part:BBa_K808000
Designed by: Valentina Herbring, Sebastian Palluk, Andreas Schmidt Group: iGEM12_TU_Darmstadt (2012-08-11)
araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter. L(+) - Arabinose is a sugar and is harmless.
For overexpression of proteins the concentration in range of 0,001-0,02% Arabinose can be used.
This sequenced biobrick was alingned by using nucleotide NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_SPEC=WGS&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch) with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.
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Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/other
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/other
biology | |
control | araC, arabinose |
direction | Forward |
n/a | Inducible pBad/araC promoter |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | 1 |