Part:BBa_K533012:Design

safety version of pSB1C3 plasmid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 2590
  Illegal XbaI site found at 2596
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2590
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2590
  Illegal BamHI site found at 2566
  Illegal XhoI site found at 1033
  Illegal XhoI site found at 1925
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal suffix found at 2
  Illegal EcoRI site found at 2590
  Illegal XbaI site found at 2596
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2590
  Illegal XbaI site found at 2596
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2146
  

Design Notes

This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common E. coli strains. In this way, the spread of this plasmid is restricted.

The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.[http://2011.igem.org/Team:Tsinghua/safety#Proposal_on_increased_safety|Safety proposal]

Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.

Source

derived from pSB1C3