Deleted
Sample Discontinued
Experience: Works
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Plasmid_Backbone

Part:BBa_K533012:Design

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-10-23)

safety version of pSB1C3 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal BamHI site found at 2566
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2146


Design Notes

This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common E. coli strains. In this way, the spread of this plasmid is restricted.

The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our [http://2011.igem.org/Team:Tsinghua/safety#Proposal_on_increased_safety| restriction-mutation system].

Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.

Source

derived from pSB1C3

References