Regulatory
Part:BBa_K622005:Experience
Designed by: Fumitaka Hashiya Group: iGEM11_Kyoto (2011-10-02)
Revision as of 17:02, 14 October 2011 by V08ken (Talk | contribs) (→Characterization of σ54 promoter activity)
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Characterization of σ54 promoter activity
We performed the function check of σ54 promoter in the genome of E. coli.
By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). The activity in 0.0 % glutamine was measured as a standard and those in 0.2 and 2.0 % glutamine were compared to the standard. Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.
After the overnight cultivation in M9 medium,
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