Part:BBa_K622005:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterization of σ54 promoter activity
We performed the function check of σ54 promoter in the genome of E. coli.
By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). The activity in 0.0 % glutamine was measured as a standard and those in 0.2 and 2.0 % glutamine were compared to the standard. Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.
After the overnight cultivation in M9 medium with glucose and casamino acids, the medium was replaced with fresh M9 medium with glutamine not containing glucose or casamino acids and the incubation at 37 degrees was started. After the 13 minutes incubation, RNA was extracted, cDNA was synthesized, and real time PCR was performed.
User Reviews
UNIQ26a89d575b9234ef-partinfo-00000000-QINU UNIQ26a89d575b9234ef-partinfo-00000001-QINU