Regulatory

Part:BBa_K622005:Experience

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-02)
Revision as of 10:24, 14 October 2011 by Daiki (Talk | contribs) (→‎Characterization of σ54 promoter activity)

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Characterization of σ54 promoter activity

We performed the function check of σ54 promoter in the genome of E. coli.

By measuring the amount of mRNA of glnG, the promoter activities in the different glutamine concentration were measured (Fig.1). Figure 1 shows that the activities in 0.2 and 2.0 % glutamine are repressed to less than one-fifth of that in 0.0 % glutamine.

Fig.1: σ54 promoter activity.

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