DNA
scar

Part:BBa_J176041:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-10-05)
Revision as of 17:26, 5 October 2011 by Kahaynes (Talk | contribs) (→‎Source)

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Silver scar


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of mRNA into the desired fusion protein. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands this does not disrupt the function of our synthetic mammalian parts.

Source

This sequence is generated when BioBricks are ligated via assembly standard 23 (Silver standard).

References

Assembly standard 23 (Silver standard) https://parts.igem.org/Silver_standard