Part:BBa_J52008:Experience

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User Reviews

UNIQ5d06c912296aa8a5-partinfo-00000000-QINU UNIQ5d06c912296aa8a5-partinfo-00000001-QINU

••••• iGEM TUDelft 2008 Ostassen

During our project of constructing an RNA thermometer to repress RNA translation we've used this part succesfully to produce luciferase. For more info check [http://2008.igem.org/Team:TUDelft/Temperature_results#Luciferase_Measurements this link]. Graphs visible over there are luciferase activity measurements.

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••••• Catramp

The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and the appropriate luciferin is added, and is capable of working with standard luciferase testing kits and luminometers. Improvements: This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier (Dual Luciferase Assay Component).

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••••• UT-Tokyo

UT-Tokyo 2011 Team has characterized that this part emits luminescence with a promoter and substrate (D-luciferin). For testing this device, we carried out a promoter assay using BBa_R0011 and BBa_J23119 as a control. We succesfully evaluated the relative expression levels of BBa_R0011 with various IPTG concentration, compared to that of BBa_J23119. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page]. Improvements: UT-Tokyo 2011 team has utilized this part to devise a Firefly-Renilla Dual Luciferase Assay Kit. This kit makes an evaluation of promoters and other cis-elements much easyer and more quantitative than existing devices (including GFP-reporter system). We also conducted a calibration of Photinus pyralis luciferase using a standard reagent, demonstrating that intracellular luciferase amount can be directly estimated from relative luminescence unit (RPU) by a linear regression. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our page].

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UNIQ5d06c912296aa8a5-partinfo-00000005-QINU