Composite

Part:BBa_K569014:Design

Designed by: David Golynskiy & Tyler Guinn   Group: iGEM11_UT_Dallas   (2011-09-27)
Revision as of 23:57, 28 September 2011 by Mtg082000 (Talk | contribs) (→‎Design Notes)

SCP+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ToxR-- Received from iGEM, part J07009

FGFR-- Received from Openbiosystems 

      Added restriction sites XbaI and SpeI via PCR mechanism
      Did a point mutation experiment because there was an EcoRI restriction site within the gene

Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119

Source

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References