Composite

Part:BBa_K569014:Design

Designed by: David Golynskiy & Tyler Guinn   Group: iGEM11_UT_Dallas   (2011-09-27)

SCP+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR 

    - 1. Added restriction sites XbaI and SpeI via PCR mechanism
    - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

ToxR-- J07009 (MIT)

FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)

Strong Constitutive Promoter-- BBa_J23119 (Berkeley)

References