Part:BBa_K118025:Experience
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how you used this part and how it worked out.
Applications of BBa_K118025
Limonene synthase expression with pRARE2 in C41 DE3 E.coli that can be used to synthesize limonene terpenes.
User Reviews
UNIQ0127e09a471b0fbc-partinfo-00000000-QINU
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British Columbia iGEM 2011 |
This composite part was expressed in our C41 E. coli that contained a pRARE plasmid encoding rare tRNA synthases. However, when we tried to express the limonene synthase in yeast, we were not able to obtain evidence for protein expression or function. So, we recommend that this part only be introduced to organisms capable of expressing foreign DNA. |
UNIQ0127e09a471b0fbc-partinfo-00000002-QINU
Characterization by British Columbia iGEM 2011
GC-MS analysis of Limonene Synthase function by British Columbia iGEM 2011
The limonene generator with lac promoter (BBa_K118025) includes the limonene generator (BBa_K118024) under the control of a lac promoter (BBa_J33207). This composite part's purpose is to express the limonene synthase (BBa_I742110), which is also available with a ribosome site (BBa_I742111).